arXiv:2410.15902physics.optics
MINFLUX -- molecular resolution with minimal photons
Early discovery. Linked by one identifiable commenter. This paper is outside the main ranking; a mention is not an endorsement.
Abstract
Optical super-resolution microscopy is a key technology for structural biology that offers high imaging contrast and live-cell compatibility. Minimal (fluorescence) photons flux microscopy, or MINFLUX, is an emerging super-resolution technique that localizes single fluorophores with high spatiotemporal precision by targeted scanning of a patterned excitation beam featuring a minimum. MINFLUX offers super-resolution imaging with nanometer resolution. When tracking single fluorophores, MINFLUX can achieve nanometer spatial and sub-millisecond temporal resolution over long tracks, greatly outperforming camera-based techniques. In this review, we present the basic working principle of MINFLUX and explain how it can reach high photon efficiencies. We then outline the advantages and limitations of MINFLUX, describe recent extensions and variations of MINFLUX and, finally, provide an outlook for future developments.
You can track things in live cells with MINFLUX, one of the recent super-resolution techniques coming from Stefan Hell's lab. Edit: add MINFLUX review: https://arxiv.org/pdf/2410.15902
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