HackerPapers

arXiv:2104.01533q-bio.GN

Unexpected novel Merbecovirus discoveries in agricultural sequencing datasets from Wuhan, China

Daoyu Zhang, Adrian Jones, Yuri Deigin, Karl Sirotkin, Alejandro Sousa

Abstract

In this study we document the unexpected discovery of multiple coronaviruses and a BSL-3 pathogen in agricultural cotton and rice sequencing datasets. In particular, we have identified a novel HKU5-related Merbecovirus in a cotton dataset sequenced by the Huazhong Agricultural University in 2017. We have also found an infectious clone sequence containing a novel HKU4-related Merbecovirus related to MERS coronavirus in a rice dataset sequenced by the Huazhong Agricultural University in early 2020. Another HKU5-related Merbecovirus, as well as Japanese encephalitis virus, were identified in a cotton dataset sequenced by the Huazhong Agricultural University in 2018. An HKU3-related Betacoronavirus was found in a Mus musculus sequencing dataset from the Wuhan Institute of Virology in 2017. Finally, a SARS-WIV1-like Betacoronavirus was found in a rice dataset sequenced by the Fujian Agriculture and Forestry University in 2017. Using the contaminating reads we have extracted from the above datasets, we were able to assemble complete genomes of two novel coronaviruses which we disclose herein. In light of our findings, we raise concerns about biosafety protocol breaches, as indicated by our discovery of multiple dangerous human pathogens in agricultural sequencing laboratories in Wuhan and Fouzou City, China.

arXiv comments
Supplementary information and data can be found in Zenodo datasets doi: 10.5281/zenodo.4660981, doi: 10.5281/zenodo.4620604, doi: 10.5281/zenodo.4399248
Journal reference
DOI
Subjects
q-bio.GNGenomics

On Hacker News

Linked by 2 identifiable commenters in 5 comments across 3 threads, from Apr 2021 to Feb 2022. Found in 1 HN submission from Apr 2021 to Apr 2021. Open submission discussions.

Comments linking this paper, per year.

Discussed in comments3 threads

    • Very few research groups in any field have published everything they're working on. Sometimes that's a deliberate choice, to avoid enabling rivals; but even when you're trying to be as open as you can, it takes time to write stuff up. The WIV has claimed that they'd published…

      tripletaoView on HNParent context

  1. The media's lab leak fiasco 1 comment
    • It seems like you have a lot more confidence than I do that we know everything the WIV was working on? I agree that SARS-CoV-2 is unlikely to be derived (naturally, artificially, or otherwise) from any known virus, including RaTG13; but a distinctive characteristic of the WIV's…

      tripletaoView on HNParent context

    • Deigin has said explicitly elsewhere that if SARS-CoV-2 arose from a lab accident, he believes it arose from manipulation of a novel, unpublished virus collected by the WIV from nature. This makes any arguments based on distance from existing, published viruses irrelevant.…

      tripletaoView on HNParent context

    • > Tons of people know what they work on and what they have in their collection. If that's true, then why has the WIV removed public access from their database? It serves only to remove a valuable scientific resource, and to cast suspicion on China; so why would they do such a…

      tripletaoView on HNParent context

    • (reposting sans downmodded comment) Here is fresh evidence (< 1 wk ago) that labs in Wuhan have worked on unpublished coronaviruses: https://arxiv.org/abs/2104.01533 The above findings are replicable by any bioinformatician operating on published sources. This preprint was…

      ricksunnyView on HNParent context

Submitted to Hacker News

Connected through shared HN threads, not inferred similarity or a recommendation.